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KMID : 0545119950050010022
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Journal of Microbiology and Biotechnology
1995 Volume.5 No. 1 p.22 ~ p.25
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Optimal conditions for pigmentation in Bacillus licheniformis SSA3 and cloning of a DNA fragment involved in pigment production
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Kim, Jong kyu
Shin, Ok Sun/Lee, Sang Jun
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Abstract
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Bacillus licheniformis SSA3 can produce a dark-brown antimutagenic pigment. The optimal conditions for production of this pigment are reached at 0.1% tyrosine, in pH 6-8, within 7-9 days, at 30℃, and in aerobic condition. We cloned a DNA fragment involved in pigment synthesis from Bacillus licheniformis SSA3 using a mutant strain. The cloned DNA was 7kb in size, which can produce the same pigment even in E. coli.
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